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CD9 (BA2, DRAP27, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP1, P24, Tetraspanin 29, Tspan29) (PE)

CD9 (BA2, DRAP27, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP1, P24, Tetraspanin 29, Tspan29) (PE)

Cat no: C2260-06E

Supplier: United States Biological
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Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Recent studies indicate CD9 may be involved with calcium mobilization. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Cultured Nalm-6 human tumor cells were washed two times. 2. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of C2260-06E at 1:50 dilution (10ug/ml). 3. Washed three times, fixed and analyzed by FACS. Cells stained with a mean shift of 2.64 log10 fluorescent units when compared to a Mouse IgG1/PE negative control at a similar concentration. Binding was 99% blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of C2260-06B. A565/A280 Ratio: 3.16 Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Catalogue number: C2260-06E
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with PE.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1. Y. Luo, et al, (1989) Cancer Research 49: 706-710. 2. F. Lanza, et al, (1991) J Biol Chem 266: 10638-10645. 3. K. Kuroda, et al, (1995) J Immunol 155: 4427-4436
Additional info: Recognizes the human tetraspan CD9 molecule of about 24kD.

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