Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Recent studies indicate CD9 may be involved with calcium mobilization.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.
1. Cultured Nalm-6 human tumor cells were washed two times.
2. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of C2260-06E at 1:50 dilution (10ug/ml).
3. Washed three times, fixed and analyzed by FACS. Cells stained with a mean shift of 2.64 log10 fluorescent units when compared to a Mouse IgG1/PE negative control at a similar concentration. Binding was 99% blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of C2260-06B.
A565/A280 Ratio: 3.16
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.