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CD84 (FITC)

CD84 (FITC)

Cat no: C2436-10C

Supplier: United States Biological
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Human CD84 is a cell surface glycoprotein found on mature B lymphocytes, monocytes, and a subset of T lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml). 80ul labels 5x10e5 Raji cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Raji cells were incubated 45 minutes on ice with 80ul of C2436-10C at a 1:50 dilution (10ug/ml). 2. They were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.81 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD84 antibody (C2436-10A). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2436-10C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.05% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 699-700, 1099, 1102. 2. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 193-195.
Additional info: Recognizes human CD84, a molecule of ~74kD.

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