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CD83 (PE) (B-cell activation protein, BL11, Cell Surface Protein HB15)

Cat no: C2435-04C

Supplier: United States Biological
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Human CD83 is a type 1 cell surface glycoprotein expressed almost solely by dendritic cells that play an important role in antigen presentation (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 human Raji cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2435-04C at a dilution factor of 1:50. 2. Cells washed three times, fixed and analyzed using a BD FACS Calibur. 3. Cells stained positive with a mean shift of 0.6 log10 fluorescent units when compared to a mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was 99% blocked when cells were pre-incubated for 10 minutes with 20ul of C2435-04 at 0.5mg/ml. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2435-04C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE)
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. L-J. Zhou, et al, (1992) J Immunol 149: 735-742. 2. L-J. Zhou & T.F. Tedder (1995) J Immunol 154: 3821-3835. 3. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 191-193.
Additional info: Recognizes the human CD83 molecule of 45kD.

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