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CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (PE)

Cat no: C2433-10D

Supplier: United States Biological
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Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion, and cellular activation (1). Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation (2, 3). Recent work with CD81-null mice indicates CD81 is important for development of humoral immune response (4). Applications: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 labels 5x10e5 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Raji cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2433-10D at a dilution factor of 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.11 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was blocked when cells were preincubated for 10 minutes with 20ul of 0.5mg/ml C2433-10A. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of enzymatic activity.
Catalogue number: C2433-10D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 684-691. 2. B.A. Mannion, et al, (1996) J Immunol 157: 2039-2047. 3. S.C. Todd, et al, (1996) J Exp Med 184: 2055-2060. 4. H.T. Maecker & S. Levy (1997) J Exp Med 185: 1505-1510.
Additional info: Recognizes the 24kD tetraspan human CD81 molecule.

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