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CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (FITC)

CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (FITC)

Cat no: C2433-10C

Supplier: United States Biological
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Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion, and cellular activation (1). Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation (2, 3). Recent work with CD81-null mice indicates CD81 is important for development of humoral immune response (4). Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml). 80ul labels 5x10e5 cultured human CEM cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human CEM cells were washed and incubated 45 minutes on ice with 80ul of C2433-10C at a 1:50 dilution factor (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD81 antibody (C2433-10A). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2433-10C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 684-691. 2. B.A. Mannion, et al, (1996) J Immunol 157: 2039-2047. 3. S.C. Todd, et al, (1996) J Exp Med 184: 2055-2060. 4. H.T. Maecker & S. Levy (1997) J Exp Med 185: 1505-1510.
Additional info: Recognzies human CD81, a 24kD tetraspan.

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