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CD8 (PE)

Cat no: C2258-91J


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-84B2 IgG2a NEGATIVE CONTROL, Murine (PE) Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C before rreconstitution. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2258-91J
Reactivities: Bovine, Goat, Sheep
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with PE. Reconstitution: Reconstitute with 1ml sterile dH2O.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. MacHugh, N.D. et al. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8). Vet. Immunol. Immunopathol. 27: 65-69. 2. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 3. Twizere, J. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo. J. Virol. 74 (21): 9895 - 9902.
Additional info: Recognizes bovine CD8. Species Crossreactivity: goat and sheep.

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