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CD8 (Leu2 T lymphocyte antigen, MAL, OKT8 T Cell antigen, p32, T cell antigen Leu2, T cell surface glycoprotein CD8 alpha, Suppressor/Cytotoxic T cell) (FITC)

CD8 (Leu2 T lymphocyte antigen, MAL, OKT8 T Cell antigen, p32, T cell antigen Leu2, T cell surface glycoprotein CD8 alpha, Suppressor/Cytotoxic T cell) (FITC)

Cat no: C2258-91M

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-83Y1 IgG2a Negative Control Murine (FITC) Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: C2258-91M
Reactivities: Bovine, Goat, Sheep
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a lliquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with FITC.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. MacHugh, N.D. et al. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8). Vet. Immunol. Immunopathol. 27: 65-69. 2. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 3. Twizere, J. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo. J. Virol. 74 (21): 9895 - 9902.
Additional info: Recognizes bovine CD8. Species Crossreactivity: goat and sheep.

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