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Cat no: C2424-01B

Supplier: United States Biological
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Human CD69 is expressed by platelets and mature thymocytes and appears rapidly after activation on T cells, B cells and NK cells. This pattern of cellular CD69 expression has suggested a lymphocyte activation role. Functional studies with antibodies to CD69 also support an activation role for CD69. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (10ug/ml) labels 5x10e5 cultured human HEL92.1 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human HEL92.1 cells were washed and pre-incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. Cells were incubate for 45 minutes on ice with 80ul of C2424-01B at a 1:50 dilution factor (10ug/ml). 3. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a mouse IgG2a/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD69 antibody (C2424-01). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2424-01B
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC). FITC:IgG ratio: 8:1.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. Ziegler, S.F., et al., Stem Cells 12: 456-465 (1994). 2. Testi, R., et al, Immunol. Today 15: 479-483 (1994). 3. Leukocyte Typing V (S.F. Schlossman, et al., eds.) Oxford University Press, Oxford, (1995) p. 1123-1132. 4. Vanhecke, D., et al., J. Immunol. 155: 1862-1872 (1995).
Additional info: Recognizes the dimeric human CD69 molecule of 60kD.

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