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CD63 (PE)

Cat no: C2418-02F


Supplier: United States Biological
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Human CD63 is expressed on activated platelets and also on monocytes and macrophages. CD63 contains four hydrophobic transmembrane regions and is a member of the tetraspan family. CD63 appears to be involved with cell adhesion and associates with VLA-3 and VLA-6 integrins. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (or use 1.6ul concentrated stock per 5x10e5 cells) Optimal dilutions to be determined by the researcher. Performance: 5x10e5 cultured HPB-MLT cells were washed and incubated 45 mins. on ice with 80ul of C2418-02F at a 1:50 dilution factor (20ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.70 log10 fluorescent units when compared to a mouse IgG1/PE negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD63 antibody (Cat. #C2418-02C). Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Catalogue number: C2418-02F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. F. Berditchevski, et al, (1995) J Biol Chem 270: 17784-17790. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1352-1364.
Additional info: Recognizes the CD63 molecule of about 50kD.

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