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CD62p (LECAM, P-Selectin, Endothelial Leukocyte Adhesion Molecule) (FITC)

Cat no: C2415-55H

Supplier: United States Biological
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Human CD62P appears on the surface of activated endothelial cells or platelets and likely is involved with regulating the recruitment of leukocytes to areas of inflammation or infection. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, and blocking the activated endothelium or platelet-neutrophil interaction. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test) Optimal dilutions to be determined by the researcher. Performance: 1. Freshly drawn heparinized whole blood was activated with 2uM TRA peptide for 10 minutes. 2. 5ul of whole blood was pre-incubated ~5 min with 20ul of human IgG at 250ug/ml (to block specific binding.) 3. 80ul of C2415-55H diluted to 1:50 (10ug/ml) in FACS buffer was added. 4. After a 45 min incubation on ice, cells were fixed with 250ul of 2% buffered paraformaldehyde and analyzed by FACS. 5. A net 70% of the gated population of platelets stained positive with a mean shift of 1.50 log10 flourescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was minimal on unstimulated platelets. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2415-55H
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Conjugated to FITC. Flourescin/IgG ratio: 7.8.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1.) G.I. Johnston, et al, (1989) Cell 56: 1033-1044. 2.) J.G. Geng, et al, (1990) Nature 343: 757-760. 3.) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1501-1501 4.) M.R. Elstad, et al, (1995) J Immunol 155: 2109-2122.
Additional info: Recognizes the protease sensitive lectin epitope of CD62P.

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