Human CD62P appears on the surface of activated endothelial cells or platelets and likely is involved with regulating the recruitment of leukocytes to areas of inflammation or infection.
Suitable for use in Flow Cytometry, Immunohistochemistry, and blocking the activated endothelium or platelet-neutrophil interaction. Other applications not tested.
Flow Cytometry: 1:50 (80ul/test)
Optimal dilutions to be determined by the researcher.
1. Freshly drawn heparinized whole blood was activated with 2uM TRA peptide for 10 minutes.
2. 5ul of whole blood was pre-incubated ~5 min with 20ul
of human IgG at 250ug/ml (to block specific binding.)
3. 80ul of C2415-55H diluted to 1:50 (10ug/ml) in FACS buffer was added.
4. After a 45 min incubation on ice, cells were fixed with 250ul of 2% buffered paraformaldehyde and analyzed by FACS.
5. A net 70% of the gated population of platelets stained positive with a mean shift of 1.50 log10 flourescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was minimal on unstimulated platelets.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.