Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Immunohistochemistry (frozen): acetone-fixed cryostat sections or cell smears.
Flow Cytometry: 1:20 as determined on RPMI8866 cells. Can be used in a 2-step procedure using the biotin-(strept)avidin system.
Optimal dilutions to be determined by researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.