Human CD62E (E-selectin) is an adhesion molecule expressed on activated endothelium and facilitates recruitment of leukocytes to sights of acute inflammation and immunological events. CD62E has affinity to LewisX (1).
Suitable for use in Flow Cytometry and blocking the function of CD62E. Other applications not tested.
Flow Cytometry: 1:50 (80ul/test)
Optimal dilutions to be determined by the researcher.
1. Cultured human umbilical cord vein endothelialcells (HUVEC) were stimulated 4 hours with 10 ng/ml Phorbol 12-Myristate 13-Acetate.
2. Five x 10e5 cells per tube were harvested by ETDA method, washed and incubated 45 minutes on ice with 80 ul of C2414-94F at a 1:50 dilution factor (20ug/ml).
3. They were then washed 3 times, fixed and analyzed on a BD FACS Calibur.
4. A net 64% population of the cells stained positive with a mean shift of 0.84 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 5. Binding was 75% blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml C2414-94D.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.