CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat, Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.
Optimal dilution determined by the researcher.
Spleen cells from immunized Balb/c mice were fused with cells of the NS1 myeloma cell line.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.