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CD60b (FITC)

Cat no: C2413-79B

Supplier: United States Biological
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Human CD60 antigen is a glycolipid determinant found on about 30% of peripheral blood T cells. The glycolipid determinant is linked to a glycoprotein which can transduce activation signals. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50; 80ul labels 5x10e5 ficoll prepared human PBL cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human PBL were incubated 45 minutes on ice with 80ul of C2413-79B at a 1:50 dilution (10ug/ml). 2. Cells were then washed three times, fixed and analyzed by FACS. A 39% sub population of the cells stained positive with a mean shift of 2.3 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. 3. Binding was blocked when cells were pre incubated for 10 minutes with 20ul of 0.5mg/ml anti-CD60b antibody (C2413-79). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2413-79B
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgM
Purity: Purified
References: 1. Higgs, J.B., et al., J. Immunol. 140: 3758-3765 (1988). 2. Fox, D.A., et al., Cell Immunol. 128: 480-489 (1990). 3. Kniep, B., et al., Blood 82: 1776-1786 (1993). 4. Rieber, E.P. & G. Rank, G., J. Exp. Med. 179: 1385-1390 (1994). 5. Leukocyte Typing V (S.F. Schlossman, et al., eds.) Oxford University Press, Oxford, p. 398-404 (1995). 6. Mason, D., et al., Leukocyte Typing VII. Oxford University Press, New York (2002).
Additional info: Recognizes human CD60b. Recognizes a 9-0-acetylated disialosyl group linked to the glycoprotein CD60. Will activiate T cells.

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