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CD59 (MACIF, MIRL, Protectin) (FITC)

Cat no: C2413-21Q

Supplier: United States Biological
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Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1). Studies are progressing to determine the regulatory mechanism for CD59 expression (2). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50. 80ul labels 5x10e5 cultured HPB-MLT cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of C2413-21Q at a 1:50 dilution factor (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.44 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD59 antibody (C2413-21N). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2413-21Q
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing V (S F Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1476-1477. 2. M H Holguin, et al, (1996) J Immunol 157: 1659-1668. 3. A E Fritzinger, F. Marciano-Cabral, et al. (2006) Infection and Immunity 74(2):1189-1195. 4. J Zhang, C Gerhardinger, M Lorenzi (2002) Diabetes 51(12): 3499-3504
Additional info: Recognizes human CD59 at 18-22kD.

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