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CD58 (LFA-3) (APC)

Cat no: C2412-06P

Supplier: United States Biological
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CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules.CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: neat-1:10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Storage and Stability: Store at 4 degrees C before opening. DO NOT FREEZE. This product is stable at 4 degrees C as a lyophilizes powder or as an undiluted liquid. Dilute only prior to immediate use.
Catalogue number: C2412-06P
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a lyophilized powder in 0.09% sodium azide and 1% BSA. Reconstitute in 1ml dH2O or 40-50% glycerol. Labeled with Allophycocyanin (APC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Makgoba, M.W. et al. (1989) Immunology Today 10:417-422. 2. Shaw, S., Johnson, J.P., (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W., et al., Oxford University Press. pp 714-716. 3. Grundy, J. E., Pahal, G.S., and Akbar, A.N. (1993) Immunology 78:413-420.
Additional info: Recognizes human CD58 at 55-70kD.

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