Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses.
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. Five x 10e5 ficoll prepared human peripheral white blood cells per tube were washed and preincubated ~5 minutes with 20 ul of 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of C2410-55F at a dilution factor of 1:50 (10ug/ml).
2. Cells were washed three times, fixed and analyzed using a BD FACS Calibur.
3. A net 13% sub population of the cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration.
4. Binding was 88% blocked when pre incubated 10 minutes with 20 ul of 0.5 mg/ml unlabeled antibody C2410-55C
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. Light sensitive.