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CD56 (NCAM-1, Neural Cell Adhesion Molecule) (PE)

Cat no: C2410-55F

Supplier: United States Biological
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Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared human peripheral white blood cells per tube were washed and preincubated ~5 minutes with 20 ul of 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of C2410-55F at a dilution factor of 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed using a BD FACS Calibur. 3. A net 13% sub population of the cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was 88% blocked when pre incubated 10 minutes with 20 ul of 0.5 mg/ml unlabeled antibody C2410-55C Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. Light sensitive.
Catalogue number: C2410-55F
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Conjugated to PE.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1. S.P. Bourne, et al, (1991) J Neuro-Oncol 10:111-119. 2. T.L. Whiteside and R.B. Herberman (1994) Clinical & Diagnostic Laboratory Immunology. 3. H. Spits, et al, (1995) Blood 85:2654-2670.
Additional info: Recognizes an epitope within the 1st four Ig-like domains of the human CD56 molecule.

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