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CD54 (Intercellular Adhesion Molecule, ICAM-1) (PE)

Cat no: C2409-10H

Supplier: United States Biological
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Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and monocytes. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of C2409-10H at a 1:50 dilution. 2. Cells were washed three times, fixed and analyzed using a BD FACstar plus. 3. Cells stained positive with a mean shift of 2.47 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was 91% blocked when pre incubated for 10 minutes with 20ul of 0.5mg/ml C2409-10E. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Catalogue number: C2409-10H
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with PE.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. A.R. Berendt, et al, (1992) Cell 68: 71-81. 2. I. Dransfield, et al, (1992) J Cell Biol 116: 1527-1535. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550. 4. P.L. Reilly, et al, (1995) J Immunol 155: 529-532.
Additional info: Recognizes the D2 domain of the human CD54 molecule of ~90kD. Inhibits CD54 binding to LFA-1

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