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CD54 (Intercellular Adhesion Molecule, ICAM-1) (PE)

Cat no: C2409-10D

Supplier: United States Biological
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Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes in weak but is upregulated on activated T and B lymphocytes and on monocytes. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Daudi cells were washed and preincubated ~5 minutes with 20ul of 0.25mg/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of C2409-10D at a dilution of 1:50. 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.78 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was 99% blocked when preincubated 10 min with 20ul of C2409-10A at a concentration of 0.5mg/ml. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2409-10D
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1. Berendt, A.R., et al., Cell 68: 71-81 (1992). 2. Dransfield, I., et al., J. Cell Biol. 116: 1527-1535 (1992). 3. Leukocyte Typing V (Schlossman, S.F., et al., eds.) Oxford University Press, Oxford, p.1548-1550 (1995). 4. Reilly, P.L., et al., J. Immunol. 155: 529-532 (1995).
Additional info: Recognizes the D1 domain of the human CD54 molecule of 90kD. Inhibits CD54 binding to LFA-1.

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