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CD54 (Intercellular Adhesion Molecule, ICAM-1) (FITC)

CD54 (Intercellular Adhesion Molecule, ICAM-1) (FITC)

Cat no: C2409-10C

Supplier: United States Biological
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Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes. Functional Application: Antibody inhibits CD54 binding to LFA-1. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2409-10C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. A.R. Berendt, et al, (1992) Cell 68: 71-81. 2. I. Dransfield, et al, (1992) J Cell Biol 116: 1527-1535. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550. 4. P.L. Reilly, et al, (1995) J Immunol 155: 529-532.
Additional info: Recognizes the D1 domain of the CD54 molecule of 90kD.

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