CD50 is also known as Inter-cellular adhesion molecule-3 (ICAM-3). CD50 is an 110kD type I transmembrane glycoprotein which is expressed mainly on resting leukocytes. It interacts with lymphocyte function-associated antigen-1 (LFA-1) and DC-SIGN, and is involved in the intercellular adhesion of leukocytes and augments CD3 signalling in T-lymphocyte activation. CD50 expression induces cancer cell proliferation and over-expression may contribute to cancer progression. It has also been implicated in limiting HIV-1 replication.
Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.
Flow Cytometry: 1:20 detected CD50 on HL-60 cells using direct staining.
Immunohistochemistry: Can be used to stain both indirectly and directly acetone-fixed cryostat sections or cell smears.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.