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CD45RO (Leukocyte Common Antigen, LCA, Ly-5 T200) (FITC)

Cat no: C2400-70C

Supplier: United States Biological
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Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. CD45RO plays an essential role in lymphocyte activation. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:40 (10ug/ml). 80ul of diluted C2400-07C labels 5x10e5 ficoll prepared human peripheral blood mononuclear cells. Immunohistochemistry: Fixed paraffin sections. Optimal dilutions to be determined by the researcher. Flow Cytometry Performance: 1. 5x10e5 ficoll prepared human peripheral blood lymphocytes were washed and pre-incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. After which they were incubated 45 minutes on ice with 80ul of C2400-70C at a 1:50 (10ug/ml) dilution. 3. Cells were washed three times, fixed and analyzed by FACS. A net 28% sub population of the cells stained positive with a mean shift of 1.05 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD45RO antibody (Catalog #C2400-70A). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2400-70C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. S.H. Smith, et al, (1986) Immunology 58: 63-70. 2. M. Streulo, et al, (1988) J Immunol 141: 3910-3914. 3. M. Merkenschlager & P.C.L. Beverly, (1989) Intl Immunol 1: 450-459. I.S. 4. Trowbridge & M.L. Thomas, (1994) Annu Rev Immunol 12: 85-116. 5. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) 6. p. 386-399. D. Leitenberg, et al, (1996) J Exp Med 183: 249-259.
Additional info: Recognizes the CD45RO isoform of about 180kD.

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