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CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (Cy5)

Cat no: C2399-14G


Supplier: United States Biological
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CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220kD) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2399-14G
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Cy5.
P type: Mab
Isotype: IgG2a
Purity: Purified
References: 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD45 Section, Academic Press, New York, p. 244. 3. Dalchau, R., J. Kirkley, and J.W. Fabre. Eur. J. Immunol. 10:737. 4. Thomas, M.L. 1989. Annu. Rev. Immunol. 7:339. 5. Trowbridge, I.S., and M.L. Thomas. 1994. Annu. Rev. Immunol. 12:85.
Additional info: Recognizes human CD45.

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