Recommended dilution is 1:20 as determined on RPMI8866 cells.
Immunohistochemistry (frozen sections, paraffin sections):
Stains indirectly or directly acetone-fixed cryostat sections, paraffin sections or cell smears; recommended is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections; use at 2-3ug/ml;
Suitable for paraffin-embedded tissue after microwave treatment (2x5 min); use at 5ug/ml;
Western Blot (see protocol)
Optimal conditions must be determined individually for each application.
Long Term storage +4 degrees C.