Home  >  Products  >  CD44V3 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (Biotin)

CD44V3 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (Biotin)

Cat no: C2398-39E


Supplier: United States Biological
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Application(s): Flow Cytometry: Suitable as primary antibody in staining for FACS analysis. Recommended concentration is 7ug/ml as determined on HACAT cells. The product can be used in a two-step procedure using the biotin-(strept-)avidin system. Recommended dilution is 1:20 as determined on HACAT cells. Immunohistochemistry: Frozen sections: Can be used to stain both indirectly or directly acetone-fixed cryostat sections or cell smears. Recommended is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), the peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. It may be used at concentration of 10ug/ml for cryostat sections. Paraffin sections: Suitable for paraffin-embedded tissue after microwave treatment. It may be used at a concentration of 10ug/ml. Western Blot (see separate protocol) Optimal conditions must be determined individually for each application.
Catalogue number: C2398-39E
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Conjugates: Biotin
Size: 100Tests
Form: Liquid. 100 tests in 1ml TRIS, pH 7.4, containing 100mM NaCl, 1% BSA and 0.02% sodium azide.
P type: Mab
Isotype: IgG1
Additional info: Recognizes an epitope encoded by exon v3 on the variant portion of human CD44.

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