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CD42b (Biotin) (Glycoprotein Ib, GPIb)

Cat no: C2395-11F

Supplier: United States Biological
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CD42b, also known as gpIb, is a two-chain membrane glycoprotein that forms a noncovalent complex with gpIX (CD42a) on the surface of platelets and megakaryocytes. Binding of the gpIb/gpIX heterodimer to von Willebrand factor bound to exposed subendothelial surfaces is essential for platelet adhesion at sites of injury.1-6 Applications: Suitable for use in Flow cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Immunohistochemistry: frozen sections and paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2395-11F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Conjugates: Biotin
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. labeled with Biotin.
P type: Mab
Isotype: IgG1
Purity: Purified
References: 1. Schlossman, S.,et al., Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.(1995). 2. Barclay, A.N., et al. The Leukocyte Antigens Facts Book, 2nd Edition, CD42a, b Section, Academic Press, New York, p. 235. (1997). 3. Thorsen, L.I., et al. . Brit. J. Haematol. 68:67. (1988). 4. Hornby, E.J., et al., . Brit. J. Haematol. 79:277. (1991). 5. Berndt, M.C.et al., Biochemistry 27:633 (1987). 6. Roth, G.J. Immunol. Today 13:100. (1992).
Additional info: Recognizes human CD42b.

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