CD40 is a type I membrane protein found on the surface of B cells and primary carcinomas. It belongs to the TNF-R family. CD40 serves as the receptor for CD40 ligand (CD40L), a cytokine expressed on the cell surface of T cells. CD40 plays a critical role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with T cells expressing CD40L, and activation of CD4+ T cells. In non-hemopoitic cells such as epidermal basal cells, CD40 ligation serves as the signal for inhibition of cell growth and induction of differentiation. The cytoplasmic domain of CD40 interacts with TRAF6, a TNFR associated factor homolog that mediates signaling from CD40. Ligation of CD40 with its ligand preferentially induces stress-activated protein kinases in B lymphocytes.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.