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CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (PE)

CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (PE)

Cat no: C2255-03P

Supplier: United States Biological
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Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and incubated for 45 minutes on ice with 80ul of C2255-03P at a dilution of 1:50 (10ug/ml). 2. Cells washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.97 log10 fluorescent units when compared to a mouse IgG2b/R-PE negative control (I1904-84H3) at a similar concentration. 4. Binding was 99% blocked when cells were pre-incubated for 10 minutes with 20ul of C2255-03L at 0.5mg/ml. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2255-03P
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.05% sodium azide. Labeld with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. Dr. E.P. Reiber. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 476-478. R. K
Additional info: Recognizes the domain 2 region of the CD4 molecule and reacts with human CD4 cell transfectants.

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