Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120.
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Flow Cytometry: 1:50 (10ug/ml)
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and incubated for 45 minutes on ice with 80ul of C2255-03P at a dilution of 1:50 (10ug/ml).
2. Cells washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.97 log10 fluorescent units when compared to a mouse IgG2b/R-PE negative control (I1904-84H3) at a similar concentration.
4. Binding was 99% blocked when cells were pre-incubated for 10 minutes with 20ul of C2255-03L at 0.5mg/ml.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.