Human CD34 is an unusual glycosylated membrane protein exclusively expressed on immature hematopoietic cells and on endothelial cells. CD34 may have a role in cell adhesion. CD34 has extensive utility for bone marrow transplantation.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry: Formalin fixed paraffin embedded sections.
Flow Cytometry: 1:50 (10ug/ml)
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. Five x 10e5 cultured human KG-1 cells per tube were washed and preincubated ~5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding).
2. After which they were incubated 45 minutes on ice with 80ul of C2386-08C at a 1:50 dilution (10ug/ml).
3. Cells were washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 1.17 log10 fluorescent units when compared to a Mouse IgG3/FITC negative control at a similar concentration.
5. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5 mg/ml C2386-08A.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
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