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CD31 (PECAM-1, Platelet gpIIa Molecule) (PE)

Cat no: C2383-01D

Supplier: United States Biological
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Human CD31 is an adhesion molecule expressed on platelets, endothelial cells, leukocytes and their bone marrow precursors. CD31 plays a role in homophilic adhesion and heterophilic transendothelial migration. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Protocol: 5 x 10e5 cultured Jurkat cells were washed and incubated 45 minutes on ice with 80ul of C2383-01D at a 1:50 dilution (20ug/ml). Cells were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.75 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was 99% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2383-01A. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Catalogue number: C2383-01D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Conjugated to R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 362-372. 2. P.J. Newman, (1997) J Clin Invest 99: 3-10. 3. K.L. Yong, et al, (1998) Blood 91: 1196-1205.
Additional info: Recognizes human CD31.

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