CD273, is also known as B7-DC and Programmed Death Ligand 2 (PD-L2). CD273 is a 42kD member of the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1). CD273 is primarily expressed on dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells. CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is
particularly important in the maintenance of self tolerance.
Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution:
Flow Cytometry: Neat Use 10ul to label 1x10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
Cells from immunized mice were fused with cells of the P3U1 myeloma cell line.
Recommended Negative Controls
Mouse IgG1 Negative Control:R-PE
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C. Reconstitute with sterile dH2O. Aliquot and store at 4 degrees C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.