CD26 is also known as dipeptidyl peptidase IV (DPP IV) and adenosine deaminase (ADA)-binding protein. It is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells. It is upregulated during T-cell activation. CD26 is also expressed on activated B cells, natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production. Antibody does not prevent ADA binding to CD26.
Suitable for use in Flow Cytometry, Immunohistochemistry,
and Immunoprecipitation. Other applications have not been tested.
Immunohistochemistry (frozen sections): use PAP, APAAP or three stage immunoperoxidase tequnique on acetone-fixed cryostat sections.
Flow Cytometry: 1:20 determined on A704 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.