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CD243 (PE)

Cat no: M2825-15C

Supplier: United States Biological
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CD243 is expressed at low levels in the cell membrane of peripheral blood leukocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumor suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell (2). Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase. Applications: Suitable for use in Flow cytometry.Other applications have not been tested. Recommended Dilution: Flow cytometry: neat; Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C before opening. DO NOT FREEZE! Reconstitute to nominal volume by adding ddH2O. Aliquot and store at 4C. Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Catalogue number: M2825-15C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a lyophilized powder from PBS, pH7.4, 1% BSA, 5% sucrose, 0.09% sodium azide,. Labeled with PE. Reconstitute with 1ml ddh2O.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatpgraphy.
References: 1. Mechetner, E.B and Roninson, I.B. (1992) Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. P.N.A.S. 89: 5824-5828. 2. Park, S.W. et al. (2003) Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay. Cytometry Part A. 53A: 67-78.
Additional info: Specifically recognizes an extracellular conformational epitope of human CD243 also known as MDR1 and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. Species crossreactivity: primate. Does not crossrect with mouse or rat.

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