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CD235a (Glycophorin A, GpA) (FITC)

Cat no: C2548-98D3

Supplier: United States Biological
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Human CD235a (glycophorin A) is a sialoglycoprotein expressed early on the cell surface during erythropoiesis as pluripotent stem cells are recruited to differentiate into erythrocytes (1). CD235a (glycophorin A) is a useful marker to study the developmental biology of hematopoietic progenitor cells (2,3). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50. 80ul labels 10x10e6 human red blood cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Wash 10x10e6 human red blood cells and preincubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. Incubated the cells for 45 minutes on ice with 80ul of C2548-98D3 at a 1:50 dilution. 3. Wash cells three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD235a (Glycophorin A) antibody (C2548-98D1). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2548-98D3
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgM
Purity: Purified (90% SDS-PAGE).
References: 1. J.G. Mark, et al, (1999) Blood 93: 4425-4435. 2. N. Debili, et al, (1996) Blood 88: 1284-1296. 3. B.M. Frey, et al, (1998) Blood 91: 2781-2792.
Additional info: Recognizes an extracellular (peptide) domain of human CD235a.

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