CD20 is a non-glycosylated phosphoprotein expressed on the surface of all mature B-cells. Membrane-spanning 4-domains, subfamily A, member 1, also known as MS4A1, is a human gene. This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. The protein has no known natural ligand and its function is unclear. It is suspected that it acts as a calcium channel in the cell membrane.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry:1:50 (10ug/ml) labels 5x10e5 Raji cells
Optimal dilutions to be determined by the researcher.
Flow cytometry Protocol:
1. Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of C2270-01K at a dilution of 1:50 (10 ug/ml).
2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.93 log10 fluorescent units when compared to a Mouse IgG2b/FITC negative control at a similar concentration.
4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5 mg/ml C2270-01H
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.