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CD1a (Langerhans Cells) (PE)

CD1a (Langerhans Cells) (PE)

Cat no: C2251-53K

Supplier: United States Biological
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The human CD1 genetic locus consists of five closely related genes. Three of the gene products (CD1a, CD1b and CD1c) have been officially clustered using antibodies and are expressed on cortical thymocytes, a subset of B cells, and on most professional antigen presenting cells. CD1a is an important marker to help identify developmental pathways of dendritic cells. CD1 molecules have a specialized lipid antigen-presenting function and it is unlikely that they present conventional peptide antigens. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of C2251-53K at 10ug/ml. 2. Cells were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.43 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5 mg/ml C2252-02J. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2251-53K
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing III (A.J. McMichael, et al, eds.) Oxford University Press, Oxford, (1987) p. 882. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 337-341. 3. E.M. Beckman, et al, (1994). 4. Nature 372: 691-694. R.S. Blumberg, et al, (1995) Immunol Rev 147: 5-29. 5. T. Ito, et al, (1999) J Immunol 163:1409-1419.
Additional info: Recognizes human CD1a at ~49kD.

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