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CD19 (FITC)

CD19 (FITC)

Cat no: C2269-83W

Supplier: United States Biological
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Human CD19 is a B cell restricted membrane protein expressed from early stages of B cell development and associates with the antigen receptor complex. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji cells were incubated 45 minutes on ice with 80ul of C2269-83W at 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.69 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of C2269-83U. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Catalogue number: C2269-83W
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Fluorescein Isothiocyanate (FITC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. D.T. Fearon & R.H. Carter, (1995) Annu Rev Immunol 13: 127-149. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 507-511. 3. S. Behr & F. Schriever, (1995) J Exp Med 182: 1191-1199.
Additional info: Recognizes human CD19 at ~95kD. Induces adhesion of B cells

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