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CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2) (FITC)

Cat no: C2269-01G

Supplier: United States Biological
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Human CD18 (beta2 integrin) forms a heterodimer with either alphaL, alphaM, or alphaX integrins (CD11a, b or c). CD11/CD18 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. Functional Application: Antibody blocks binding of ICAM-1 and ICAM-3 to LFA-1 (2, 5). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2269-01G
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. S.D. Wright et al, (1983) Proc Natl Acad Sci USA 80: 5699-5703. 2. R.C. Landis et al, (1994) J Cell Biol 126: 529-537. 3. M. Kaneko, et al, (1995) J Immunol 155: 2631-2641. 4. A. Mazzone & G. Ricevuti, (1995) Haematologica 80: 161-175.
Additional info: Recognizes the beta 2 integrin subunit (CD18) adhesion molecule of about 95kD.

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