Receptors that recognize the Fc portion of IgG are divided into three groups designated Fcg RI, RII, and RIII, also known respectively as CD64, CD32 and CD16. In humans, each group of receptors, is encoded by 2-3 closely related genes designated A, B, and C. Fcg RI binds IgG with high affinity and functions during early immune responses. Fcg RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Different Fc receptors can function as activators, as inhibitors, or as decoy receptors.
Suitable for use in ELISA. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.