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CD16 (Fc gamma RIII alpha, NK cells) (PE)

Cat no: C2267-33D

Supplier: United States Biological
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Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphtidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen sections. Flow Cytometry: 1:50 for 5x10e5 cells. Optimal dilutions to be determined by researcher. Flow Cytometry Protocol 1. Wash 5 x10e5 cells of ficoll prepared human peripheral blood lymphocytes. 2. Incubate on ice for 45 minutes with 80ul of C2267-33D at 1:50 dilution (10ug/ml). 3. Wash 3 times, fix and analyze by FACS. 4. An 25% sub-population of the cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to a mouse IgG1(PE) negative control at a similar concentration. 5. Binding was blocked when cells were pre-incubated for 10 min. with 20ul of 0.5 mg/ml anti-CD16 antibody (C2267-33A). Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2267-33D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79: 3275-3279. 2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597. 3. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12: 218-234. 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814. 5. J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.
Additional info: Recognizes both allelic forms of human CD16. Blocks binding of complexed IgG to CD16.

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