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CD16 (Fc gamma RIII alpha, NK cells) (Biotin)

Cat no: C2267-29F


Supplier: United States Biological
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CD16, a member of the immunoglobulin superfamily, is a 50-65kD glycoprotein found as both a transmembrane and GPI-linked form. The transmembrane form of CD16 is expressed on NK cells, granulocytes, macrophages, and mast cells but not on eosinophils. The GPI-anchored type of CD16 is found only on neutrophils. CD16 is involved in NK activation and signal transduction. Applications: Suitable for use Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2267-29F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugates: Biotin
Size: 100Tests
Form: Supplied as a liquid in PBS, sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG2a
Purity: Purified
References: 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD16 Section, Academic Press, New York, p. 173. 4. Ravetch, J.V., and B. Perussia. 1989. J. Exp. Med. 170:481. 5. Takai, T., M. Li, D. Sylvestre, R. Clynes, and J.V. Ravetch. 1994. Cell 76:519.
Additional info: Recognizes human CD16.

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