Home  >  Products  >  CD15 (Lewis x, Le x, Hematopoietic Differentiation Antigen, SSEA-1, Stage-speci?c Embryonic Antigen, X Hapten, 3-fucosyl-N-acetyllactosamine, 3-FAL) (PE)

CD15 (Lewis x, Le x, Hematopoietic Differentiation Antigen, SSEA-1, Stage-speci?c Embryonic Antigen, X Hapten, 3-fucosyl-N-acetyllactosamine, 3-FAL) (PE)

Cat no: C2266-01F


Supplier: United States Biological
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CD15 is a carbohydrate antigen (also known as Lewis X antigen) found on the cell surface of neutrophils, eosinophils and monocytes, but not on basophils, platelets, lymphocytes or erythrocytes. CD15 may function in adhesion as its ligand is L-selectin (CD62L).1-5 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Inhibition of neutrophil phagocytosis. Optimal dilutions to be determined by the researcher. Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2266-01F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.
P type: Mab
Isotype: IgM
Purity: Purified
References: 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford. 3. Hogg, N., S. MacDonald, M. Slusarenko, and P.C. Beverley. 1984. Immunol. 53:753. 4. Stocks, S.C., M. Albrechtsen, and M.A. Kerr. 1990. Biochem. J. 268:275. 5. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD15 Section, Academic Press, New York, p. 171.
Additional info: Recognizes human CD15.

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