Home  >  Products  >  CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 antigen, 5F7, Blood-brain barrier HT7 antigen, Collagenase stimulatory factor, Emmprin, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, TCSF, Tumor cell der

CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 antigen, 5F7, Blood-brain barrier HT7 antigen, Collagenase stimulatory factor, Emmprin, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, TCSF, Tumor cell der

Cat no: C2548-80T


Supplier: United States Biological
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Human CD147 is a transmembrane glycoprotein expressed more intensely on thymocytes than on mature peripheral blood T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human Raji cells were washed and incubated for 45 minutes on ice with 80ul of C2548-80T at 10ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat x mouse. 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2548-80T
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate before the addition of glycerol to 40%.
P type: Mab
Isotype: IgG1,k
Purity: >95% (SDS-PAGE). Purified by Protein A affinity chromatography.
References: 1. T. Kishimoto, et al, eds., Leukocyte Typing VI Garland Publishing, Inc., New York (1997) p. 760-766. 2. A.H. Kirsch, et al, (1997) Tissue Antigens 50:147-152.
Additional info: Recognizes the human CD147 molecule of about 35-40kD. Inhibits homotypic aggregation, adhesion to matrix proteins and migation through matrigel.

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