Human CD147 is a transmembrane glycoprotein expressed more intensely on thymocytes than on mature peripheral blood T cells.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 10ug/ml
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. 5x10e5 cultured human Raji cells were washed and incubated for 45 minutes on ice with 80ul of C2548-80T at 10ug/ml.
2. Cells were washed twice and incubated with IgG (FITC) goat x mouse.
3. Cells were washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.