Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival.
Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Flow Cytometry: 1:50, 80ul/test
Optimal dilutions to be determined by the researcher.
Antibody blocks binding of CD137 huIg to Raji cells
Fluorescein/IgG Molar Ratio: 9.8
Cultured human CEM were stimulated 2 days in the presence of PMA (10ng/ml) and ionomycin (1mM). 5 x 10e5 cells were washed and incubated 45 minutes on ice with 80ul of C2548-39K, 1:50 Cells were washed three times, fixed and analyzed on a BD FACstar plus. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was 85% blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD137 antibody C2548-39F.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.