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CD134 (OX40) (PE)

Cat no: C2515-19C

Supplier: United States Biological
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Human CD134 (OX40) is an activation-associated antigen which is predominantly expressed on activated CD4 positive cells. CD134 antigen is a member of the tumor necrosis factor (TNF) receptor family of molecules and may be involved with regulating T cell-dependent B cell proliferation and differentiation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (5ug/ml) labels 5x10e5 cultured HPB-MLT human tumor cells Optimal dilutions to be determined by the researcher. Flow cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2515-19C at a 5ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.85 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD134 antibody (C2515-19). Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2515-19C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry
Size: 120Tests
Form: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% Glycerol. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. U. Latza et al, Eur J Immunol (1994) 24: 677-683. 2. E. Stuber, et al, (1995) Immunity 2: 507-521. 3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 464-465. 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1157-1160.
Additional info: Recognizes the human CD134 molecule of about 35kD.

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