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CD134 Ligand (CD134L, OX40L, CD252) (FITC)

Cat no: C2515-40C

Supplier: United States Biological
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Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (5,6,7,8). Applications: Suitable for use in Flow Cytometry, ELISA and blocking studies. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (or 1.6ul neat/5x10e5 cells/test) Blocking: Blocks binding of Recombinant CD134 to CD134L on HUVEC. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells (HUVEC) were harvested by trypsinization. 5x10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of C2515-40C at a 1:50 dilution (10ug/ml). 2. Washed three times, fixed and analyzed on a BD FACS Calibur A. Binding was 87% blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml C2515-40A. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2515-40C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with FITC. Molar Ratio: 8.1 FITC /IgG.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2. L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3. A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826. 4. V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 5. V. Malmstrom, et al, (2001) J Immunol 166: 6972. 6. C. Nohara, et al, (2001) J Immunol 166: 2108-2115. 7. X. Yuan, et al, (2003) J Immunol 170: 2949-2955. 8. L. Tian, et al, (2002) Transplantation 74: 133-138.
Additional info: Binds to CD134L on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.

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