Home  >  Products  >  CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (PE)

CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (PE)

Cat no: C2446-71J


Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Human CD106 is an endothelial adhesion molecule that binds to a4 b1 & a4b7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells. Applications: Suitable for use in Flow Cytometry and blocks lymphocyte adhesion to CD106.. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelialcells were stimulated 4 hours with 10ng/ml Phorbol 12-Myristate 13-Acetate. 2. Five x 10e5 cells per tube were harvested, washed and incubated 45 minutes on ice with 80ul of C2446-71J at a 1:50 dilution. 3. Cells were washed three times, fixed and analyzed on a BD FACstar plus. 4. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was 93% blocked when pre incubated 10 minutes with 20ul of 0.5 mg/ml of C2446-71F. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Catalogue number: C2446-71J
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in 50mM Sodium phosphate pH 7.5, 500mM potassium chloride, 150mM NaCl,, 0.2% BSA, 0.04% sodium azide, 15% glycerol.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. M.H. Thornhill, D.O. Haskard, (1991) J Immunol 146: 592-598. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767. 3. M. Nagata, et al, (1995) J Immunol 155: 2194-2202. 4. H.E. Chuluyan, et al, (1995) J Immunol 155: 3135-314.
Additional info: Recognizes the VCAM-1 molecule (CD106) which is involved with leukocyte adhesion.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave