Human CD106 is an endothelial adhesion molecule that binds to a4 b1 & a4b7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells.
Suitable for use in Flow Cytometry and blocks lymphocyte adhesion to CD106.. Other applications not tested.
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. Cultured human umbilical cord vein endothelialcells were stimulated 4 hours with 10ng/ml Phorbol 12-Myristate 13-Acetate.
2. Five x 10e5 cells per tube were harvested, washed and incubated 45 minutes on ice with 80ul of C2446-71J at a 1:50 dilution.
3. Cells were washed three times, fixed and analyzed on a BD FACstar plus.
4. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was 93% blocked when pre incubated 10 minutes with 20ul of 0.5 mg/ml of C2446-71F.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.