The kit contains nonradioactive components for assessing the histone acetyl transferase (HAT) activity of immunoprecipitated p300/CBP.
Quantity:
40 assays per kit using 5ug of immunoprecipitating antibody per assay.
Kit Components:
C2098-07A: p300; 1x200ug Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05%
sodium azide.
C2098-07B: Histone H4 Peptide (Biotin) 1x00ug
Supplied as a lyophilized powder. Reconstitute in 500ul distilled water.
C2098-07C: HAT Assay Buffer (5X); 1x2ml
Supplied as a liquid in 250mM Tris-HCl, pH 8.0, 50% glycerol, 0.5mM EDTA and 5mM dithiothreitol.
C2098-07D: P81 Phosphocellulose Squares; 1x200 prelabeled squares.
Storage and Stability:
Store at -20 degrees C. Stable for 12 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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