This kit is optimized to detect Cathepsin D activity. Enhanced Value: It provides enough reagents to perform 100 assays in a 96-well format.
High Speed: The entire process can be completed in one hour
Cathepsin D is the lysosomal aspartic proteinase implicated in intracellular protein degradation. It is involved in several pathological processes, such as inflammatory states, atherosclerosis, thrombosis, apoptosis, neoplastic proliferation or Alzheimer disease.
This kit provides a convenient assay for screening of enzyme inhibitors and for continuous assay of Cathepsin D activity using a Mca/Dnp FRET peptide. The sequence of this FRET peptide is derived from the cleavage site of Cathepsin D. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330nm/390nm.
C2097-33A: Cathepsin D substrate (1.6mM), Ex/Em=330nm / 390nm upon cleavage, 1x50ul
C2097-33B: Standard (2mM,), Ex/Em=330nm/390nm, 1x10ul
*C2097-33C: Cathepsin D, bovine spleen (0.1mg/ml), 1x20 ul
C2097-33D: Assay Buffer, 20ml
C2097-33E: Pepstatin A (4mM), 1x100ul
C2097-33F: DTT (1M), 1x100ul
Storage and Stability:
Store *C2097-33C at -70 degrees C. Store other components at 20 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.