Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47kD) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37kD/17kD) and a small subunit (10kD). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry: 10-20ug/ml
Immunocytochemistry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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